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HORIZON-HLTH-2023-STAYHLTH-01-01

HORIZON-HLTH-2023-ENVHLTH-02-01

HORIZON-HLTH-2023-ENVHLTH-02-02

HORIZON-HLTH-2023-ENVHLTH-02-03

HORIZON-HLTH-2023-CARE-04-02

IMMOBILIARE LPC SRL

info@clinicofiorenzuola.it
IT

The aims of CLINICO are Health Promotion and Disease Prevention in the Health Care System through individual health treatments in preventing people from developing any kind of diseases. One of the key points of this health facility is “sustainability”, that mean to improve the quality of life of people in taking into consideration also future generations. CLINICO aims to be the local reference point for a wide range of health and paramedical activities, aimed at protecting health in general, obtaining prompt diagnosis, carrying out specific therapies and the general well-being of the person. Furthermore, it aims to be an important point of reference for the quality of the services provided, customer satisfaction and professional hospitality.

HORIZON-HLTH-2024-DISEASE-03-13 HORIZON-HLTH-2023-TOOL-05-05 HORIZON-HLTH-2023-TOOL-05-08

National Institute of Chemistry

barbara.tisler@ki.si

 

SI

Accurate quantification of low abundance biomarkers remains difficult when relying on ensemble techniques, especially when working with complex samples such as various body fluids. Our laboratory seeks to address this issue using nanopore biosensing, a single-molecule technique that allows quantification of analytes at the femtomolar level. In contrast to direct identification, where Expertise / Commitment offered analytes are detected by current traces as they pass through the nanopore, our approach is based on modular nanopores in which the protein nanopore is covalently bound to a ligand of choice (biomarker-specific aptamer or nanobody). The protein nanopore is produced with an unnatural amino acid that can undergo click chemistry, allowing us to use a range of different ligands. The analytes, i.e., the biomarkers, are then detected and quantified based on the current changes that arise from complex formation between the immobilized ligand and the analyte. In this way, we ensure specific detection of the analyte and eliminate the need for translocation of the analyte. In addition to biomarkers, our laboratory is continuously developing a variety of useful protein nanopores suitable for in situ detection of other low abundance compounds, such as pollutants or toxins.

HORIZON-HLTH-2024-TOOL-5.06

National Institute of Chemistry

barbara.tisler@ki.si

SI

With the appearance of new treatment strategies for complex maladies which are driven by various physiological factors, there is an urgent need for the development of innovative tools to monitor the effectiveness and toxicity of newly established treatments, offer hands-on instruments for investigation of such treatments and applications as well as provide a resource for the basic research. Expertise / Commitment offered In our group, we are focused on developing and utilizing synthetic biology to tackle pressing issues of biomedical research and we are especially interested in establishing novel cancer immunotherapies. Complex 3D in vitro models that closely recapitulate the tumor microenvironment are necessary for the successful development and safety assessment of novel drugs, including immunotherapies. Our aim is to develop multicellular 3D cancer in vitro models comprised of various human cell types, most importantly including a plethora of immune cells. As a proof of concept, we plan to recapitulate the melanoma microenvironment, stemming from established cell lines and primary cells. These models will be easily adjustable and just changing the ratios between different cell types will result in a change of the properties of the tumor microenvironment, allowing us to test new therapeutic approaches in various conditions. Infrastructure The Department of Synthetic Biology and Immunology at NIC Slovenia has access to diverse infrastructure, including several microscopes (confocal, highthroughput, SEM, TEM and cryoEM) and flow cytometers, which will enable the characterization of prepared 3D cancer models and the effectiveness of developed treatments

HORIZON-HLTH-2023-ENVHLTH-02-03

HORIZON-HLTH-2024-TOOL-05-06

National Institute of Chemistry

barbara.tisler@ki.si

SI

Short introduction of key areas of institute’s research: The National Institute of Chemistry is a scientifically excellent, established and breakthrough research institution in Europe and the second largest natural sciences research institute in Slovenia with 374 employees (31. 12. 2021), of which around 331 carry out research work in 9 departments and two infrastructure centers. Through our cutting-edge research, we are enriching the global treasury of knowledge and working together to solve the most pressing challenges faced by society. Health, sustainable energy, climate change, circular economy and safe food are the most important among them. We measure our research goals in surpluses that push the boundaries of science and create new values. We successfully transfer knowledge to the industrial environment, thus supporting the placement of science in societal development in the long run.

HORIZON-HLTH-2024-DISEASE-08-20

National Institute of Chemistry

barbara.tisler@ki.si

SI

Research in our group is focused on host-pathogen interactions at the molecular level. We are interested in pathogens that affect either humans, animals or plants, and are of microbial origin (bacteria, oomycetes, fungi as well as viruses). We have vast experience in identification and characterization of molecular Expertise / Commitment offered interactions at the biochemical and biophysical level, and determine interactions at the structural (atomic level), to describe molecular mechanism of action. We have ample experiences in studying molecular interactions between different species of biological (macro) molecules (proteins, lipids, nucleic acids, sugars – and hybrids between them). Especially we are experienced in protein-lipid interactions, which are in many cases a driving force in pathogen-host interactions. Using our knowledge and expertise we could contribute to areas addressed in the call: identification and characterization of receptors on the host cell , identification and characterization of viral surface proteins that are capable of interacting with host target cells, and characterization of the mechanism of viral uptake in the host cell with regard to the topology and the dynamics of the host receptor – virus ligand interaction, identification of receptor and ligand (sub)units that could be targeted by preventive or therapeutic intervention.

HORIZON-HLTH-2023-DISEASE-03-07

National Institute of Chemistry

barbara.tisler@ki.si

SI

Over the past decade, numerous studies demonstrated the involvement of innate immune pathways in driving inflammation in non-communicable diseases such as diabetes, rheumatoid arthritis and it is likely that the same pathways contribute to severe COVID19 and long COVID19 symptoms. Particularly interesting in this respect might be inflammasomes, particularly NLRP3 inflammasome, which is the main topic of our research in the past Expertise / Commitment offered decade. It is known that NLRP3 needs two signals for its activation where the priming signal is usually provided by TLR agonist that could be provided by pathogens. The second signal could be provided by pathogens or even endogenous damage-associated patterns e.g. amyloid or oxidized phospholipids. Additionally, MyD88 and NF-kB signaling are not involved only in pro-inflammatory signaling, but also modulate mitochondrial dynamics and metabolic pathways. Within this project, we could participate with mechanistic studies defining how pathogens influence the development of noncommunicable diseases by working on preclinical models of multiple sclerosis/diabetes/rheumatoid arthritis and primary cells of animal and human origin. We plan to define the crucial pathways involved, specify the stages that are the most important and target those processes by de novo developed synthetic biology tools. Infrastructure The Department of Synthetic Biology and Immunology at NIC Slovenia has access to diverse infrastructure, including several microscopes (confocal, highthroughput). We have BSL2 cell culture laboratories and an animal facility, where we are currently utilizing pseudoviral systems mimicking BSL3 pathogens.